Effects of laser solid forming of porous titanium on proliferation of osteoblast and RANKL/OPG expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1278-1284, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-815097
ABSTRACT
To evaluate the effect of laser solid forming (LSF) of porous titanium on receptor activator of NF-κB ligand (RANKL)/osteoprorotegerin (OPG) expression and osteoblast cells growth.
Methods:
The DMEM and sterile saline were used for porous titanium extract. The osteoblast cells were cultured in the extract while equal amount of DMEM and sterile saline were added to the control group. The growth of the cells were observed under an inverted phase contrast microscope. MTT was used to detect the growth inhibitory rates. The adhesion capacity of osteoblasts were measured. The growth in the material surface was examined by the electron microscope, and the expressions of RANKL and OPG were determined by Westen blot.Results:
At the first day, the osteoblast proliferation rate was significantly different (P0.05); at each time point, the osteoblast proliferation rate were significantly different between the two groups (P0.05). The laser solid forming of porous titanium showed well bone compatibility.Conclusion:
The porous titanium did not affect osteoblast proliferation due to its well bone compatibility. It did not affect the OPG/RANKL/RANK-axis system of bone metabolism, exibiting a wide applicable prospect for tissue engineering.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteogênese
/
Farmacologia
/
Titânio
/
Materiais Biocompatíveis
/
Adesão Celular
/
Células Cultivadas
/
Química
/
Porosidade
/
Meios de Cultura
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2016
Tipo de documento:
Artigo
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