Identification of miR-126 knockdown mouse and the change of blood glucose / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 12-17, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-815218
ABSTRACT
OBJECTIVE@#To detect the expression of miR-126 in different tissues and organs and the change of peripheral blood glucose in microRNA-126 knock down (miR-126 KD) mouse, and to explore the pathological significance.@*METHODS@#Total RNAs were isolated from twelve kinds of tissues and organs in wild-type mouse (WT) and miR-126 KD mouse respectively. Th en, the expression level of miR-126 was detected by real-time PCR assay. Th e levels of peripheral blood glucose and body weight of miR-126 KD mice were measured. Th e pathologic changes of pancreas and lung tissue were observed by HE staining.@*RESULTS@#Compared with the WT mice, the relative expression of miR-126 in spleen, liver, muscle and lung from the miR-126 KD mice were dramatically decreased respectively (P<0.05). The level of peripheral blood glucose in the miR-126 KD mouse increased significantly at seven week and sixteen week after the birth (P<0.05). HE staining showed that the pathological structure of pancreas and liver were abnormal. The body weight of miR-126 KD mice was increased obviously from thirteen week after birth (P<0.05).@*CONCLUSION@#Peripheral blood glucose levels in the miR-126 KD mouse were dramatically elevated, which might be related to the pathological changes in the structure of pancreas and liver. These results suggest that miR-126 may play an important role in the metabolism of blood glucose and the development of type 2 diabetes mellitus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pâncreas
/
Patologia
/
Glicemia
/
Peso Corporal
/
MicroRNAs
/
Diabetes Mellitus Tipo 2
/
Técnicas de Silenciamento de Genes
/
Reação em Cadeia da Polimerase em Tempo Real
/
Genética
/
Fígado
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2015
Tipo de documento:
Artigo
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