MicroRNA-33a regulates the invasion of cervical cancer cells via targeting Twist1 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1060-1067, 2015.
Article
em Zh
| WPRIM
| ID: wpr-815225
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To examine the expression of Twist1 in cervical cancer and to explore its biological function in the progression of cervical cancer.
@*METHODS@#The expressions of Twist1 in 32 cervical cancers and matched normal tissues were examined by immunohistochemistry (IHC). Cell invasive ability and the expression of invasion-related genes were determined in RNAi-based Twist1-silencing HeLa cells. The relationship between Twist1 and microRNA-33a (miR-33a) in cervical cancer was studied by Pearson correlation analysis, and the roles of miR-33a in regulation of Twist1 and cell invasiveness were studied.
@*RESULTS@#The positive expression rate of Twist1 was 75.0% (24/32) and 21.9% (7/32) in the cervical cancer and the matched normal tissues, respectively, with significant difference between them (P<0.05). Twist1 shRNA significantly decreased the invasiveness of HeLa cells (P<0.05). Compared with the matched normal tissues, the expression of miR-33a was increased in the cervical cancer tissues, which was negatively correlated with Twist1 (r=-0.661, P<0.05). Overexpression of miR-33a could significantly suppress Twist1 expression as well as cell invasiveness (P<0.05).
@*CONCLUSION@#Twist1 is critical for the invasiveness of cervical cancer cells; miR-33a, as a tumor suppressor gene, functions as an upstream regulator of Twist1 and is involved in the invasiveness of cervical cancer cell.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Proteínas Nucleares
/
Células HeLa
/
Regulação Neoplásica da Expressão Gênica
/
Neoplasias do Colo do Útero
/
Genes Supressores de Tumor
/
MicroRNAs
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Proteína 1 Relacionada a Twist
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2015
Tipo de documento:
Article