Effect of miR-342-3p on chemotherapy sensitivity in triple-negative breast cancer / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 488-495, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-815414
ABSTRACT
OBJECTIVE@#To study the effect of miR-342-3p on the chemotherapy sensitivity in triple-negative breast cancer (TNBC).@*METHODS@#Tissue samples were collected from January 2011 to August 2013 samples in Jiangsu Cancer Hospital from a total of 32 triple-negative breast cancer patients with preoperative chemotherapy, with 5 cases of complete response (CR) and 27 cases of partial response (PR). We detected miR-342-3p expression of TNBC with RT-PCR. We transfected has-miR-342-3p mimic and inhibitor into breast cancer cell lines MDA-MB-231 by lipofection transfection and set up negative control mim-NC and inhi-NC. Group of mimic, mim-NC, inhibitor and inhi-NC were cultivated with 2 μmol /L paclitaxel, cisplatin or 4 μmol/L doxorubicin for 48 h. The cell growth rates were measured by CCK8 reagent kit, and the cell apoptosis rate by flow cytometry.@*RESULTS@#The expressions of miRNA-342-3p in TNBC tissue of CR were higher than those of PR. The cell growth rates of mimic were lower and cell apoptosis rates were higher than those of min- NC after cultivating with paclitaxel or cisplatin for 48 h, with significant difference (P0.05).@*CONCLUSION@#TNBC with high expression of miR-342-3p are more sensitive to chemotherapy. miRNA-342-3p may regulate the sensitivity of breast cancer cell line MDA-MB-231 to chemotherapy drugs paclitaxel and cisplatin, but can not affect the chemotherapy sensitivity of doxorubicin.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Transfecção
/
Doxorrubicina
/
Cisplatino
/
Paclitaxel
/
Apoptose
/
Resistencia a Medicamentos Antineoplásicos
/
MicroRNAs
/
Linhagem Celular Tumoral
/
Proliferação de Células
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2014
Tipo de documento:
Artigo
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