GPI-PLD inhibits the growth of hepatoma cells by down-regulation of PI3K-Akt signaling pathway / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 873-878, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-815509
ABSTRACT
OBJECTIVE@#To clarify the effect of glycosylphosphatidylinositol-specific phospholipase D (GPIPLD) on hepatoma cells HepG2 and the possible molecular mechanism.@*METHODS@#MTT, fluorescent staining and Western blot were applied to analyze the effect and molecular mechanism of GPI-PLD on hepatoma cells by transfected high expression GPI-PLD model. We inoculated HepG2 in nude mice models to further clarify the effect of GPI-PLD on hepatoma cells in vivo.@*RESULTS@#Compared with the control groups, PI3K-Akt signaling pathway activity and proliferation of hepatoma cells were significantly inhibited in the GPI-PLD group. Nude mice models showed that the tumor growth and tumor weight [(1.87 ± 0.09) g] of the GPI-PLD group were significantly less than those of the blank control group [(2.20 ± 0.17) g] and the negative control group [(2.15 ± 0.09) g]. AST, ALT and AFP serum concentration in the GPI-PLD group were significantly lower than those of the control groups (P<0.05).@*CONCLUSION@#GPI-PLD can inhibit the proliferation of hepatoma cells and growth in vivo, and promote the apoptosis of hepatoma cells by reducing the activity of PI3K-Akt signaling pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fosfolipase D
/
Transfecção
/
Transdução de Sinais
/
Regulação para Baixo
/
Linhagem Celular
/
Apoptose
/
Carcinoma Hepatocelular
/
Fosfatidilinositol 3-Quinases
/
Proteínas Proto-Oncogênicas c-akt
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2014
Tipo de documento:
Artigo
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