Analysis of unique long 16 binding protein 1 expression in peripheral blood exosomes of pregnant women with preeclampsia / 中国实用妇科与产科杂志

ABSTRACT

OBJECTIVE: To investigate the expression of unique long 16 binding protein 1(ULBP1)in peripheral blood exosomes of patients with preeclampsia(PE).METHODS: Twenty patients with PE who were admitted to the First Affiliated Hospital of China Medical University from June 2017 to July 2018 were enrolled.The normal control group(NC group)consisted of 20 normal pregnant women,all of whom were primiparas,singleton.Blood samples were taken from the median vein of the fasting elbow.The serum was separated by low-speed centrifugation.Extraction of exosomes from serum was performed using ExoQuick Precipitation kit,and the exosome marker protein CD63 was detected by Western blot,the morphology of exosomes were identified by transmission electron microscopy,and the expression of ULBP1 in exosomes was detected by enzyme-linked immunosorbent assay.RESULTS: The expression of ULBP1 in peripheral blood exosomes was significantly higher in PE than in the control group[(1.0500±0.5937)μg/L vs.(0.4875±0.4346)μg/L,P=0.0013(P<0.05)].CONCLUSION: The expression of ULBP1 in peripheral blood exosomes of PE patients is increased,and its increase may provide a basis for the diagnosis and treatment of PE.