Analysis of Metabolites of Jolkinolide B in Rats by UPLC-Q-TOF/MS / 中国药房
China Pharmacy
;
(12): 2796-2801, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-817523
ABSTRACT
OBJECTIVE: To analyze the metabolites of jolkinolide B in rats, and predict its metabolism pathway. METHODS: The rats were randomly divided into blank group (0.5% CMC-Na, ig) and administration group (jolkinolide B, ig, 100 mg/kg), with 8 rats in each group. The fecal samples were collected at >0-12, >12-24, >24-36 hours after administration; the urine samples were collected at >0-2, >2-8, >8-12, >12-24, >24-36, >36-48 hours after administration; the blood samples were collected at 1, 2, 8, 12, 24, 36 hours after administration. UPLC-Q-TOF/MS combined with Analyst® TF 1.7.1 and PeakView® 2.2 software were used to analyze and identify the metabolites in the samples after treated with ultrasonic extraction, solid phase extraction and protein precipitation. RESULTS & CONCLUSIONS: Prototype drugs and seven metabolites were detected in rat’s fecal samples, and one or two metabolites were detected in urine and blood samples, respectively. After intragastric administration, the metabolism of jolkinolide B in rats is mainly through ring opening, oxidation, dehydration, deoxygenation and hydrogenation of phase Ⅰ, but no phase Ⅱ metabolites were detected.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2019
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS