Dexmedetomidine Receptor Dependently Enhanced Macrophage Phagocytosis / 中山大学学报(医学科学版)
Journal of Sun Yat-sen University(Medical Sciences)
; (6): 268-272, 2020.
Article
em Zh
| WPRIM
| ID: wpr-817705
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WPRO
ABSTRACT
@#【Objective】To investigate the effect of dexmedetomidine on the phagocytosis of macrophages.【Methods】 RAW264.7 cells were divided into control group,DEX group,and BRL44408 + DEX group. Expression of α2A adrenergic receptor,p-Akt and Akt were detected by Western Blotting;Phagocytosis Assay Kit(IgG PE)was used to measure the phagocytosis of macrophages. 【Results】 α2A adrenergic receptor was detected in RAW264.7 cells;Dexmedetomidine could enhance the phagocytosis of macrophages(P < 0.001),and BRL44408 reversed the enhancement of phagocytic ability of macrophages(P < 0.001);Dexmedetomidine upregulated the expression of Akt in RAW264.7 cells,while the use of BRL44408 inhibited the activation of the Akt pathway(P < 0.01).【Conclusion】Dexmedetomidine could enhance phagocytosis of RAW264.7 by activating the Akt pathway through the α2A adrenergic receptor.
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Idioma:
Zh
Revista:
Journal of Sun Yat-sen University(Medical Sciences)
Ano de publicação:
2020
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Article