Application of alveolar lavage fluid mNGS in the etiological diagnosis of children with severe pneumonia / 中国实用儿科杂志

ABSTRACT

OBJECTIVE: To explore the application of alveolar lavage fluid NGS in etiological diagnosis of children with severe pneumonia. METHODS: The clinical data of 34 children with severe pneumonia hospitalized in Pediatric Respiratory Department fromSeptember 2018 toMarch2019 wereretrospectivelyanalyzed. The collectedalveolarlavagefluidwasanalyzed bymetagenomicsNGS. RESULTS: Totally 34 caseswerediagnosedasseverepneumonia. Theresultsof m NGS:32 cases(94.1%)were detected in 34 samples,19 cases(55.9%)of Mycoplasma pneumoniae,6 cases(17.6%)of human adenovirus type 7,1 case(2.9%)of Forsystanella,1 case of Staphylococcus aureus(2.9%);1 cases(2.9%)was not detected,and the positive rate of Mycoplasma pneumoniae was the highest. The mixed infection showed human adenovirus type 7+ Mycoplasma pneumoniae in 2 cases(5.9%),human adenovirus type 7+ Haemophilus influenzae in 1 case(2.9%)and human adenovirus type 7+ Aspergillus fumigatus in 3 cases(8.8%). After anti-infection and symptomatic support treatment,33 cases were improved and discharged,and 1 case died. CONCLUSION: The metagenomics NGS can improve the diagnostic accuracy of the pathogen in severe pneumonia,and early and accurate identification of the pathogen plays an important role in guiding the rational use of antibiotics in clinical practice.