Study on the induction of mononuclearleukemia cell THP-1 drived macrophages by S component of Panton-Valentine leukocidin in vitro / 医学研究生学报

ABSTRACT

Objective　There are few studies on the in vitro induction of human leukemia cell line THP-1 macrophages by Staphylococcus aureus PV leukin S component (LukS-PV). This study aims to investigate whether LukS-PV could induce the polarization of Human THP-1 derived macrophage in vitro.　Methods　THP-1 monocytes were induced to macrophages by Phorbol myristate acetate (PMA). The cell surface markers CD11b and CD14 were detected by flow cytometry at 48h and 72h after the induction, respectively. Using 0.1, 0.2 and 0.4 μmol/LlukS-PV treated THP-1 macrophages as the 0.1 μmol/LLukS-PV group, 0.2 μmol/LLukS-PV group, and 0.4 μmol/LLukS-PV group, respectively. The THP-1 driven macrophage induced by PMA was used as the control group. Flow cytometry was used to detect the expression of CD80 and CD206 in each group at 24h and 48h. The mRNA expression levels of IL-12, TNF-α and TGF-β were detected by real-time fluorescence-based quantitative PCR (qRT-PCR).　Results　The expression of CD80 on the cell surface in the 0.1 μmol/LLukS-PV group, 0.2 μmol/LLukS-PV group and 0.4 μmol/LLukS-PV significantly increased (P <0.01), with the highest expression in the 0.2 μM LukS-PV group (P<0.01), when comparing to that of the control group. The mRNA expressions of IL-12 and TNF-α in the 0.2 μM LukS-PV group significantly increased (7.32±0.91 and 5.29±0.51, respectively) at 24h (P <0.01), and raised in 0.2 μM LukS-PV group (11.16±0.78、6.70±0.68) at 48h (P <0.01), when comparing to those of control group (0.71±0.11 and 0.73±0.14, respectively). Compared with the control group, expression of CD80 on cell surface in the 0.2 μM LukS-PV group significantly increased at 24h and 48h (P <0.000).　Conclusion　LukS-PV could stimulate the polarization of human THP-1 macrophages toward M1.