Photothermal therapy against breast cancer 4T1 cells using PEGylated gold triangular nanoparticles / 医学研究生学报

ABSTRACT

Objective To observe the photothermal therapeutic effect of PEGylated gold triangular nanoparticles (PEG-GTN) to breast cancer 4T1 cells upon 606-nm laser irradiation. Methods The obtain gold triangular nanoparticles were functionalized with SH-PEG and incubated with 4T1 cells. The cellular toxicity and photothermal therapeutic effect of PEG-GTN to 4T1 cells were measured by MTT assays. Results After incubated with 5, 10. 20 and 40 µg/mL PEG-GTN for 24 h, the cellular viability were (96.2±4.4)%,(95.9±4.4)%,(95.2±4.8)% and (96.6±4.7)%. There were no statistical differences between PEG-GTN treated groups and control group. With the irradiation of 660-nm laser irradiation, the cellular viability of 4T1 cells were (92.2±6.2)%,(51.6±6.8)%,(25.7±4.5)% and (4.8±2.5)%. The cells treated with PEG-GTN with concentrations equal or higher than 10 µg/mL showed significant lowered cell viability. Conclusion PEG-GTN has low cytotoxicity and concentration-dependent photothermal effect.