Protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment against Pneumocystis carinii in mice
Asian Pacific Journal of Tropical Medicine
;
(12): 353-356, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-819507
ABSTRACT
OBJECTIVE@#To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii (P. carinii) against P. carinii in mice.@*METHODS@#The fragment of the antigen within p55(p55-582) was cloned. Then recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+). BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-p55-582. ELISA and RT-PCR were used to evaluate the role of this kind of DNA vaccine.@*RESULTS@#The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582] could be expressed correctly and had antigenicity in transfected COS-7 cells. ELISA and RT-PCR showed that pcDNA3.1(+)-p55-582 elicited antibody production, stimulated lymphocyte proliferation and provided partial protection by reducing the P. carinii burden.@*CONCLUSIONS@#The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Vacinas Virais
/
Chlorocebus aethiops
/
Imunização
/
Células COS
/
Vacinas de DNA
/
Pneumocystis carinii
/
Alergia e Imunologia
/
Reação em Cadeia da Polimerase em Tempo Real
/
Genética
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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