Your browser doesn't support javascript.
loading
Protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment against Pneumocystis carinii in mice
Asian Pacific Journal of Tropical Medicine ; (12): 353-356, 2011.
Artigo em Inglês | WPRIM | ID: wpr-819507
ABSTRACT
OBJECTIVE@#To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii (P. carinii) against P. carinii in mice.@*METHODS@#The fragment of the antigen within p55(p55-582) was cloned. Then recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+). BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-p55-582. ELISA and RT-PCR were used to evaluate the role of this kind of DNA vaccine.@*RESULTS@#The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582] could be expressed correctly and had antigenicity in transfected COS-7 cells. ELISA and RT-PCR showed that pcDNA3.1(+)-p55-582 elicited antibody production, stimulated lymphocyte proliferation and provided partial protection by reducing the P. carinii burden.@*CONCLUSIONS@#The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaio de Imunoadsorção Enzimática / Vacinas Virais / Chlorocebus aethiops / Imunização / Células COS / Vacinas de DNA / Pneumocystis carinii / Alergia e Imunologia / Reação em Cadeia da Polimerase em Tempo Real / Genética Limite: Animais Idioma: Inglês Revista: Asian Pacific Journal of Tropical Medicine Ano de publicação: 2011 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaio de Imunoadsorção Enzimática / Vacinas Virais / Chlorocebus aethiops / Imunização / Células COS / Vacinas de DNA / Pneumocystis carinii / Alergia e Imunologia / Reação em Cadeia da Polimerase em Tempo Real / Genética Limite: Animais Idioma: Inglês Revista: Asian Pacific Journal of Tropical Medicine Ano de publicação: 2011 Tipo de documento: Artigo