Effects of HMGA2 on malignant degree, invasion, metastasis, proliferation and cellular morphology of ovarian cancer cells
Asian Pacific Journal of Tropical Medicine
;
(12): 289-292, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-819686
ABSTRACT
OBJECTIVE@#To analyze effects of high mobility group AT-hook 2 (HMGA2) on malignant degree, invasion, metastasis, proliferation and cellular morphology of ovarian cancer cells.@*METHODS@#Three methods were applied to observe the effect on HMGA2 expression in ovarian cancer cells and ovarian epithelial cells.@*RESULTS@#After the application of siRNA-HMGA2, number of T29A2-cell clones was decreased, there was significant difference compared with the negative control Block-iT. After application of let-7c, number of T29A2+ cell clones was decreased significantly, however, after the application of Anti-let-7, the number of clones restored, and there was no significant difference compared with the negative control group. After interference, the number of T29A2- cells which passed through Matrigel polycarbonate membrane were significantly lower than the negative control group. After the treatment of siRNA-HMGA2, let-7c and sh-HMGA2 respectively, growth and proliferation of T29A2-, T29A2+ and SKOV3 were slower, and the phenomenon was most obvious in SKOV3. Stable interference of HMGA2 induced mesenchymal-epithelial changes in the morphology of SKOV3-sh-HMGA2.@*CONCLUSIONS@#HMGA2 can promote malignant transformation of ovarian cancer cells, enhance cell invasion and metastasis, and promote cell growth and proliferation of ovarian cancer cells, which can cause ovarian cancer to progress rapidly and affect the quality of life.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Patologia
/
Fisiologia
/
Proteína HMGA2
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Processos de Crescimento Celular
/
Forma Celular
/
Genética
/
Metabolismo
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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