Expression of hENTl and ERCC1 genes in tumor tissues non-small cell lung cancer
Asian Pacific Journal of Tropical Medicine
; (12): 908-911, 2013.
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ABSTRACT
OBJECTIVE@#To investigate the expression of hENTl and ERCC1 genes in tumor tissues non-small cell lung cancer (NSCLC).@*METHODS@#Fresh non-small lung cancer specimens were transplanted into nude mice. Twenty mice were randomized into two groups: experimental group receiving gemcitabine plus cisplatin and control group receiving 0.9% physiological saline. The expressions of hENTl and ERCC1 mRNA in tumor tissue were detected by real-time fluorescent quantitative PCR. The volume of tumor, the weight of nude mice and tumor volume were respectively measured and calculated 2-3 times per week. Tissue samples were collected from NSCLC mice treated with gemcitabine plus carboplatin.@*RESULTS@#The histological examination showed that many tumor cells were well preserved in nude mice. The rate of transplanted tumor cells was 86.7%. The concomitant treatment study showed that the rate of TV, RTV, T/C in GEM + DDP group was the lowest. LBP + DOC, DDP + DOC obviously influenced the body weight. Compared with NS group, DDP group, GEM group, the survival period and the level of hENTl of DDP+GEM group increased obviously, the level of ERCC1 decreased significantly (P<0.05).@*CONCLUSIONS@#The expression of hENT1 and ERCC1 genes in tumor tissues were closely correlated with the response to chemotherapy and prognosis of patients with NSCLC treated with gemcitabine plus cisplatin.
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Assunto principal:
Patologia
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RNA Mensageiro
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Protocolos de Quimioterapia Combinada Antineoplásica
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Taxa de Sobrevida
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Cisplatino
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Carcinoma Pulmonar de Células não Pequenas
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Transportador Equilibrativo 1 de Nucleosídeo
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Usos Terapêuticos
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Desoxicitidina
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Carga Tumoral
Limite:
Animals
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2013
Tipo de documento:
Article