Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus
Asian Pacific Journal of Tropical Medicine
;
(12): 355-357, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-819768
ABSTRACT
OBJECTIVE@#To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion.@*METHODS@#Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography. Four young rabbits were selected and challenged intradermally with yielded recombinant antigens. Rabbits' sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection.@*RESULTS@#The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection.@*CONCLUSIONS@#Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Vacinas Protozoárias
/
Echinococcus granulosus
/
Equinococose
/
Alergia e Imunologia
/
Formação de Anticorpos
/
Antígenos de Helmintos
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS