Bioinformatic analysis for structure and function of TCTP from Spirometra mansoni
Asian Pacific Journal of Tropical Medicine
;
(12): 709-712, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-819978
ABSTRACT
OBJECTIVE@#To predict structure and function of translationally controlled tumor protein (TCTP) from Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study.@*METHODS@#Open reading frame (ORF) of EST sequence from Spirometra mansoni was obtained by ORF finder and was translated into amino acid residue by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools Protparam, InterProScan, protscale, SignalP-3.0, PSORT II, BepiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2, the structure and function of the protein were predicted and analyzed.@*RESULTS@#The results showed that the EST sequence was Sm TCTP with 173 amino acid residues, theoretical molecular weight was 19 872.0 Da. The protein has the closest evolutionary status with Clonorchis sinensis, Schistosoma mansoni, and Schistosoma japonicum. Then it had no signal peptide site and transmembrane domain. Secondary structure of TCTP contained two α -helices and eight β -strands.@*CONCLUSIONS@#Sm TCTP was a variety of biological functions of protein that may be used as a vaccine candidate molecule and drug target.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Filogenia
/
Spirometra
/
Sequência de Bases
/
Biomarcadores Tumorais
/
Proteínas de Helminto
/
Infecções por Cestoides
/
Química
/
Fases de Leitura Aberta
/
Sequência de Aminoácidos
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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