Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid
Asian Pacific Journal of Tropical Medicine
;
(12): 473-480, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-820018
ABSTRACT
OBJECTIVE@#To identify target genes of transcription factor CCAAT enhancer-binding protein β (CEBPB) in acute promyelocytic leukemia cells induced by all-trans retinoic acid.@*METHODS@#A new strategy for high-throughput identification of direct target genes was established by combining chromatin immunoprecipitation (ChIP) with in vitro selection. Then, 106 potential CEBPB binding fragments from the genome of the all-trans retinoic acid (ATRA)-treated NB4 cells were identified.@*RESULTS@#Of them, 82 were mapped in proximity to known or previously predicted genes; 7 were randomly picked up for further confirmation by ChIP-PCR and 3 genes (GALM, ITPR2 and ORM2) were found to be specifically up-regulated in the ATRA-treated NB4 cells, indicating that they might be the down-stream target genes of ATRA.@*CONCLUSIONS@#Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Tretinoína
/
Sítios de Ligação
/
Leucemia Promielocítica Aguda
/
Transdução de Sinais
/
Regulação Neoplásica da Expressão Gênica
/
Diferenciação Celular
/
Linhagem Celular
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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