Bioinformatics analysis on ORF1 protein of Torque teno virus (SANBAN isolate)

ABSTRACT

OBJECTIVE@#To analyze the sequence of ORF1 protein of Torque teno virus to prepare for the future hybrid experiments.@*METHODS@#The sequence of ORF1 protein of Torque teno virus was analyzed by bioinformatics using some web tools.@*RESULTS@#The most likely cleavage site was between position 14aa and 15aa and signal peptide may be position 1aa-14aa. Two possible transmembrane helices from inside to outside and three possible transmembrane helices from outside to inside were found. The position 509 (NKTN) was the potential N-glycosylation site. The speculative molecular weight of TTV ORF1 protein, which may be a kind of unstable protein was 88 705.7 Da. 1aa-91aa and 278aa-361aa were localized in non-regular secondary structure region.@*CONCLUSIONS@#TTV ORF1 protein may be a nuclear protein which contains two non-regular secondary structure region. 265aa to 486aa and 510aa to 679aa may be the two approciate fragments to construct the plasmids, which would be prepared for the future hybrid experiments to study the functional positions of the protein and the interactions between TTV and its hosts. Bioinformatics analysis would possibly make it easier to study the protein's function.