Pokemon reduces Bcl-2 expression through NF-κ Bp65: A possible mechanism of hepatocellular carcinoma
Asian Pacific Journal of Tropical Medicine
;
(12): 492-497, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-820106
ABSTRACT
OBJECTIVE@#To investigate the relationship among Pokemon, NF-κ B p65 and Bcl-2 in hepatoma cells.@*METHODS@#HCC cell HepG2, SMMC7721 and human fetal liver cell line LO2 cells were used, and expression of Pokemon, NF-κ B p65 and Bcl-2 in three cells were detected by real-time PCR and western blot. Then siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-κ B p65 and apoptotic rate was determined by flow cytometric analysis.@*RESULTS@#Expressions of Pokemon, NF-κ B p65 and Bcl-2 in human hepatoma cell HepG2, SMMC7721 expression were significantly higher than those in human embryonic stem cells LO2. siRNA of Pokemon inhibited the expression of Pokemon, NF-κ B p65 and Bcl-2 in liver cancer cells, and significantly increased apoptosis of liver cells. While siRNA of NF-κ B p65 inhibited the expression of NF-κ B p65 and Bcl-2, but Pokemon expression in hepatoma cells had no significant change.@*CONCLUSIONS@#The proto-oncogene Pokemon can inhibit P14ARF by specific transcription regulation of cell cycle and can induce tumors. In addition, Pokemon can regulate NF-κ B p65 through the expression of apoptosis repressor, and promote the development of liver cancer. It suggests signal network in the liver include the regulation of new non-classical NF-κ B regulatory pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Fatores de Transcrição
/
RNA Mensageiro
/
Apoptose
/
Carcinoma Hepatocelular
/
Proteínas Proto-Oncogênicas c-bcl-2
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Proteínas de Ligação a DNA
/
Fator de Transcrição RelA
Limite:
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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