Effect of Kupffer cells on immune tolerance in liver transplantation
Asian Pacific Journal of Tropical Medicine
;
(12): 970-972, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-820584
ABSTRACT
OBJECTIVE@#To observe the effect of Kupffer cells on immune tolerance in liver transplantation.@*METHODS@#The rats were randomly divided into A, B and C groups. A group was sham operation group. The donor rats of group B had intraperitoneal injection of 1 nmol Kuppffer cells every other day for three days before liver transplantation. Rats of group C were injected with equal saline. The rat liver transplantation models were established by modified Kamada's two-cuff technique. The rats were sacrificed after 24 hours. The concentrations of ALT and AST in serum were measured with the biochemical analyzer. The level of IL-2 and TNF-α in serum were measured by ELISA method. The apoptotic indexes were detected by immunohistochemical assay.@*RESULTS@#The concentration of ALT, AST, IL-1 and TNF-α in A, B and C groups were increased successively. The levels of group C were significantly higher than that of group B and A (P<0.05), and the levels of group B were significantly higher than that of group A (P<0.05). The apoptotic indexes of three groups were 3.40±0.37, 14.70±2.54 and 26.33±3.65, respectively, with significant difference among three groups (P<0.05).@*CONCLUSIONS@#Pretreatment with Kupffer cells can reduce liver injury and raise liver transplantation immune tolerance.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Aspartato Aminotransferases
/
Transplante
/
Sangue
/
Distribuição de Qui-Quadrado
/
Química
/
Transplante de Fígado
/
Interleucina-1
/
Fator de Necrose Tumoral alfa
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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