Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast
Asian Pacific Journal of Tropical Medicine
;
(12): 557-561, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-820683
ABSTRACT
OBJECTIVE@#To explore tissue factor (TF) expression and methylation regulation in differentiation of human embryonic stem cells (hESCs) into trophoblast.@*METHODS@#Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4 (BMP4). Expression of gene, protein of TF and DNA methylation at different time points during induction process was detected by RT-PCT, Western blot, flow cytometry and MSP-PCR method.@*RESULTS@#The expression of mRNA, protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells, semi methylation-semi non methylation expression appeared at TF DNA promoter region, and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of TF.@*CONCLUSIONS@#It shows that during differentiation of hESCs into trophoblast, the differential expression of TF is related with DNA methylation level, and it is changed with the methylation or non methylated degree. It provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Trofoblastos
/
Tromboplastina
/
Diferenciação Celular
/
Linhagem Celular
/
Metilação de DNA
/
Células-Tronco Embrionárias
/
Proteína Morfogenética Óssea 4
/
Genética
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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