DAPT suppresses the proliferation of human glioma cell line SHG-44
Asian Pacific Journal of Tropical Medicine
; (12): 552-556, 2014.
Article
em En
| WPRIM
| ID: wpr-820684
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the suppressing effect of γ-secretase inhibitor DAPT on proliferation of human glioma cell line SHG-44 in vitro and its mechanism.@*METHODS@#The SHG-44 cell was treated by DAPT with different concentration. The proliferation of cells was detected by MTT assay; cell cycle and TSC of CD133(+) were determined by flow cytometry analysis technique; the key factor in Notch signaling pathway (Notch-1, Delta-1, Hes-1) was measured by reverse transcriptase-polymerase chain reaction and western blotting.@*RESULTS@#DAPT inhibited the growth and proliferation of SHG-44 cells significantly(P<0.05). And the inhibiting effect on SHG-44 cells produced by DAPT showed a dose-dependent manner. DAPT increased the rate of cells in G0/G1 phase of SHG-44 cells, while it decreased the rate of cells in S phase. TSC of CD133(+) was significantly reduced after DAPT treated SHG-44 cells. The expression of protein and mRNA of Notch-1, Delta-1 and Hes-1 were gradually downregulated with the increase of DAPT doses.@*CONCLUSIONS@#DAPT can downregulate these key factor in Notch signaling pathway, reduce the TSC of CD133+ and inhibit the proliferation of SHG-44 cells.
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Assunto principal:
Farmacologia
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Transdução de Sinais
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Ciclo Celular
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Linhagem Celular Tumoral
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Proliferação de Células
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Forma Celular
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Dipeptídeos
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Glioma
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2014
Tipo de documento:
Article