Tissue tropism and parasite burden of Toxoplasma gondii RH strain in experimentally infected mice
Asian Pacific Journal of Tropical Medicine
;
(12): 521-524, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-820689
ABSTRACT
OBJECTIVE@#To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR.@*METHODS@#In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day (before death), different tissues of mice including blood, brain, eye, liver, spleen, kidney, heart and muscle were harvested. Following tissues DNA extraction, the parasite burden was quantified using real time QPCR targeting the B1 gene (451 bp).@*RESULTS@#It showed that Toxoplasma after intraperitoneal injection was able to movement to various tissues in 24 hours. Parasite burden was high in all tissues but the most number of parasites were observed in kidney, heart and liver, respectively.@*CONCLUSIONS@#These data provide significant baseline information about Toxoplasma pathogenesis, vaccine monitoring and drug efficiency.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Toxoplasma
/
Virulência
/
Toxoplasmose
/
Reação em Cadeia da Polimerase
/
Tropismo
/
Modelos Animais de Doenças
/
Carga Parasitária
/
Coração
/
Interações Hospedeiro-Parasita
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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