Molecular determination and genotyping of Cryptosporidium spp. in fecal and respiratory samples of industrial poultry in Iran
Asian Pacific Journal of Tropical Medicine
;
(12): 517-520, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-820690
ABSTRACT
OBJECTIVE@#To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran.@*METHODS@#A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing.@*RESULTS@#From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi.@*CONCLUSIONS@#In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Doenças das Aves Domésticas
/
Traqueia
/
Galinhas
/
Reação em Cadeia da Polimerase
/
Epidemiologia
/
Prevalência
/
DNA de Protozoário
/
Criptosporidiose
/
Cryptosporidium
Tipo de estudo:
Estudo de prevalência
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS