Molecular epidemiology and characterization of Streptococcus mutans strains in Korea / 대한구강보건학회지
Journal of Korean Academy of Oral Health
;
: 34-40, 2020.
Artigo
em Inglês
| WPRIM
| ID: wpr-820819
ABSTRACT
OBJECTIVES:
We investigated the characteristics of Streptococcus mutans in the national culture collection from Korea. Twenty-nine (dental plaque, n=27; endodontic infections, n=1; blood, n=1) isolates were included in this study.METHODS:
Antimicrobial susceptibilities were tested using the disk diffusion test. Multilocus sequence typing (MLST), serotyping, and collagen-binding genes were used for polymerase chain reaction (PCR) and direct sequencing. A collagen-binding (to assess the adhesion properties) assay was performed. S. mutans demonstrated high susceptibility to antimicrobial agents. Differences in collagen-binding abilities of the cnm-positive and -negative groups were compared using the Mann-Whitney U test (P<0.05).RESULTS:
MLST analyses revealed 25 sequence types (STs), 17 of which (ST213-ST229) contained new alleles. The strains were classified into four serotypes with the c type encompassing 79.3% of all strains, while the e, f, and k types representing 6.9% each. Analysis of the cnm and cbm genes, which encode the two surface adhesin components of S. mutans, revealed three cnm-positive strains, each displaying greater adhesion ability than those of the cnm-negative strains.CONCLUSIONS:
This study highlights the presence of a wide variety of S. mutans genotypes in Korea. These findings may provide useful information regarding the pathogenesis of infectious diseases, such as dental caries.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Streptococcus
/
Streptococcus mutans
/
Sorotipagem
/
Reação em Cadeia da Polimerase
/
Doenças Transmissíveis
/
Bacteriemia
/
Epidemiologia Molecular
/
Cárie Dentária
/
Difusão
/
Alelos
Tipo de estudo:
Estudo de rastreamento
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Korean Academy of Oral Health
Ano de publicação:
2020
Tipo de documento:
Artigo
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