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Study on in vitro Antioxidant Activities of Different Polar Parts of Ethanol Extract from Amomum tsao-ko / 中国药房
China Pharmacy ; (12): 953-956, 2020.
Artigo em Chinês | WPRIM | ID: wpr-820843
ABSTRACT
OBJECTIVE:To evaluate in vitro antioxidant activities of 4 different polar parts of ethanol extract from Amomum tsao-ko,and to lay a foundation for the research and development of antioxidant chemical components in the plant. METHODS : The dried fruits of A. tsao-ko were crushed ,then were hearted and reflux extracted with 95% ethanol. The extraction fluid was concentrated by rotary evaporation and evaporated in water bath to obtain the ethanol extract. The extract was dispersed in water , and then extracted with petroleum ether ,ethyl acetate and n-butanol organic solvents one by one. Each solvent extract was combined and the lower water phase were collected. Finally ,the petroleum ether part ,ethyl acetate part ,n-butanol part and water part were obtained,after rotary evaporation concentration and water bath evaporation. Through in vitro antioxidant activity tests ,using 2, 6-di-tert-butyl-4-methylphenol(BHT)as positive control ,DPPH radical ,superoxide anion radical scavenging ability and Fe 3+ reducing ability of different polar parts of ethanol extract from A. tsao-ko were investigated. RESULTS :The scavenging rates of 4 polar parts of ethanol extract from A. tsao-ko on DPPH radical were all over 80%;the order of scavenging ability was ethyl acetate part>BHTn-butanol part >petroleum ether part >water part. Those of the 4 polar parts to superoxide anion radical were between about 30%-40% mostly;the order of scavenging ability was n-butanol part >petroleum ether part >water part >ethyl acetate part > BHT;but those were weaker than their scavenging ability to DPPH r adical. The polar parts of ethanol extract also had a certain reduction ability to Fe 3+;the order of the reduction ability was n-butanol part >BHT>ethyl acetate part >petroleum ether part > water part on the whole ,but that of water part rose to the stron- gest when its concentration was 4.0 μg/mL. CONCLUSIONS: The different polar parts of ethanol extract from A. tsao-kom have certain in vitro antioxidant capacity ,but the order of antioxidant activity of different polar parts was not the same in different antioxidant activity tests ;ethyl acetate part has the 163.com strongest scave nging effect on DPPH radical ,n-butanol part has the strongest scavenging ability on superoxide anion radical and reducing ability on Fe 3+.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2020 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2020 Tipo de documento: Artigo