Emodin assay in Ampelopsis radix by RP-HPLC / 药学实践杂志
Journal of Pharmaceutical Practice
;
(6): 264-267, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-821483
ABSTRACT
Objective To establish a method for Emodin assay in Ampelopsis radix, and to improve the quality control of Ampelopsis radix. Methods Reversed phase high performance liquid chromatography was used. The column Apollo-C18 (4.6 mm×250 mm, 5 μm); mobile phase methanol0.2% phosphoric acid (8515); flow rate 1.0 ml/min; column temperature room temperature; detection wavelength 220 nm. Results With the specified chromatographic conditions, there was a good linear relationship for Emodin in Ampelopsis radix in the range of 0.124-3.968 μg/ml. The linear regression equation was Y= 53 962X–966.46, r=0.999 7. The average recovery was 99.7%. RSD=2.5% (n=9). Conclusion This method is accurate, sensitive and repeatable. It is suitable for the determination of emodin in Ampelopsis radix.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Pharmaceutical Practice
Ano de publicação:
2020
Tipo de documento:
Artigo
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