Ovarian cancer cells promote glycolysis metabolism of peripheral CD4 + T cells / 临床检验杂志

ABSTRACT

Objective@#To investigate the effects of ovarian cancer cells on the glycolysis metabolism and functions of peripheral CD4 + T cells. @*Methods@#We established a coculture system of human CD4 + T cells with ovarian cancer cell SKOV3, and the mRNA expression levels of 8 glycolysis-related genes (mTORC1, HIF1α, GLUT1, GPI, ENO1, PKM2, LDHα and PDK1) in cocultured CD4 + T cells were determined by real-time fluorescence quantitative PCR. The levels of glucose uptake and glycolysis were detected by colorimetry. The proportion of CD4 + CD25 + Foxp3 + T cells was tested by flow cytometry. The proliferation levels of CD4 + T cells were measured by tritium incorporation method. The expression levels of 8 glycolysis-related genes in peripheral CD4 + T cells were determined by real-time fluorescence quantitative PCR in 10 ovarian cancer (OC) patients, 10 benign ovarian tumor (BOT) patients and 10 healthy control (HC). @*Results@#Results: Compared with the CD4 + T cells in single culture without SKOV3, the expression levels of 8 glycolysis-related genes in CD4 + T cells cocultured with SKOV3 were elevated (all P＜0.01). The levels of glucose uptake and glycolysis were increased (P＜0.05). The proportion of CD4 + CD25 + Foxp3 + T cells was up-regulated (P＜0.05) and the proliferation levels of CD4 + T cells were decreased (P＜0.05). Moreover, the mRNA expression levels of all the glycolysis-related genes were increased (all P＜0.05) in the peripheral CD4 + T cells of OC patients compared with BOT patients and HC. @*Conclusion@#Ovarian cancer cells could promote the glycolysis metabolism of CD4 + T cells, which may be relevant to its differentiation and functions.