Protective effects of Rhein on cardiomyocyte induced with H2O2 / 医学研究生学报

ABSTRACT

ObjectiveThe effect of Rhein on myocardial cell injury and its possible mechanism of action remains unknown. This study aims to explore the effect and mechanism of rhein on cardiomyocyte injury induced by hydrogen peroxide (H2O2).MethodsRat cardiomyocyte H9c2 was stimulated using H2O2 to establish the myocardial cell injury model. The experiment was divided into three groups including a control group, H2O2 induced group, and Rhein treated group. Fluorescence probe and biochemical methods were used to detect the oxidative stress level of H9c2 cells. RT-qPCR and immunofluorescence tests were adapted to determine the apoptosis of H9c2 cells. Besides, RT-qPCR was carried out to evaluate the inflammatory response of H9c2 cells. Finally, western blot assay was performed to evaluate the expression of MAPK and NF-κB signaling pathways.ResultsThe results of fluorescence probe showed that the expression of ROS of H9c2 cells induced by H2O2 was decreased significantly after Rhein intervention. Besides, the biochemical test showed that the expressions of GSK-Px and CAT of H9c2 cells induced by H2O2 were increased significantly after Rhein treatment. RT-qPCR data suggested that Rhein could significantly decrease the mRNA expressions of Bax, caspase-3 and caspase-9, and upregulate the mRNA expression of Bcl-2 of H9c2 cells induced by H2O2. Moreover, immunofluorescence experiments showed that Rhein could significantly inhibit the expression of caspase-3 of H9c2 cells induced with H2O2. Further, the results of RT-qPCR indicated that the mRNA expressions of IL-6, TNF-α、IL-1β and IL-1α of H9c2 cells induced by H2O2 was decreased significantly after Rhein intervention. Finally, western blot showed that Rhein can significantly inhibit the expressions of MAPK and NF-κB signaling pathways.ConclusionRhein can reduce the injury of cardiomyocytes induced by H2O2, which may be achieved by inhibiting the expressions of MAPK and NF-κB signaling pathways.