Expression of Notch signaling pathway in temporomandibular osteoarthritis / 口腔疾病防治
Journal of Prevention and Treatment for Stomatological Diseases
; (12): 143-152, 2017.
Article
em Zh
| WPRIM
| ID: wpr-822608
Biblioteca responsável:
WPRO
ABSTRACT
Objective@# To investigate the expression of Notch signaling molecules in temporomandibular joint arthritis (TMJOA), and to explore the role and mechanism of Notch signaling pathway in the development and progression of TMJOA. @*Methods @#72 Kunming mice were randomly divided into experimental group, sham-surgery group and normal group. In experimental group, discs on the right TMJ were subjected to total discectomy, sham-operation group underwent the same procedure without disc removal, while normal group serve as blank control. All the left temporomandibular joint discs were not treated. 8 mice in each group were sacrificed respectively at 1 week, 2 week, 4 week after surgery. Histological examinations were performed to assess success of TMJOA model, according to the pathological standard of osteoarthritis diagnosis. Immunochemistry techniques were performed in the successful TMJOA cartilages to evaluate the expression levels of Notch1 (NICD1), Jagged1, Hes1 and Hes5. The scores were evaluated by semi-quantitative method. @*Results @#Notch1 (NICD1), Jagged1 and Hes5 were activated in the experimental group with the expression levels increased dramatically over time. While Hes1 expression was suppressed at the beginning of osteoarthritis but was up-regulated afterwards.@*Conclusion @# Notch pathway-related molecular expression changed greatly in TMJOA model, indicating that the pathway in the occurrence and development of TMJOA plays an important role.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Journal of Prevention and Treatment for Stomatological Diseases
Ano de publicação:
2017
Tipo de documento:
Article