Effect of deferoxamine on hyperoxic lung injury in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of deferoxamine on hyperoxic lung injury in rats.Methods Twenty-four adult male Sprague-Dawley rats,weighing 170-230 g,were divided into 3 groups (n=8 each) using a random number table method:hyperoxia group (group H),deferoxamine group (group D) and air control group (group C).Mechanical ventilation was performed after tracheal intubation,group C inhaled air,and H and D groups inhaled 90％ oxygen.Deferoxamine 50 mg · kg-1 · h-1 was continuously infused via the tail vein for 4 h via the tail vein at the same time of mechanical ventilation in group D.The equal volume of normal saline was infused in H and C groups.At 4 h of mechanical ventilation,lungs were removed for examination of pathological changes of lung tissues and for determination of wet/dry weight ratio (W/D ratio) and levels of pulmonary surfactant protein C (SP-C),xanthine oxidase (XOD) and glutathione reductase (GR) in lung tissues and broncho-alveolar lavage fluid (BALF) were detected by enzyme-linked immunosorbent assay.Results Compared with group C,the pathological score of lung tissues and W/D ratio were significantly increased,the levels of SP-C and GR in lung tissues and BALF were decreased,and the level of XOD in lung tissues and BALF was increased in H and D groups (P＜0.05).Compared with group H,the pathological score of lung tissues and W/D ratio were significantly decreased,the levels of SP-C and GR in lung tissues and BALF were increased,and the level of XOD in lung tissues and BALF was decreased in group D (P＜0.05).Conclusion Deferoxamine can inhibit oxidative stress response and alleviate hyperoxic lung injury in rats.