Effect of ticagrelor on plasma P-selectin concentration during acute kidney injury in septic rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of ticagrelor on plasma P-selectin(CD62P)concentra-tion during acute kidney injury in septic rats.Methods Thirty SPF healthy male Sprague-Dawley rats,aged 6-8 weeks,weighing 180-220 g,were divided into 3 groups(n＝10 each)using a random number table method: sham operation group(group S),sepsis group(group Sep)and ticagrelor group(group T).Sepsis was induced by cecal ligation and puncture in anesthetized rats.The abdomen was opened after anesthesia in-duction and then closed after turning the intestines in group S.Ticagrelor 8.6 mg/kg was given through a gas-tric tube into stomach at 12 h after establishing the model in group T,while the equal volume of distilled wa-ter was given instead in S and Sep groups.Blood samples were collected from hearts at 24 h after establishing the model for determination of the concentrations of Scr and plasma CD62P.The rats were then sacrificed,and bilateral renal tissues were taken for examination of pathological changes and for determination of cell ap-optosis by fluorescence microscopy after Annexin/V-FITC double staining was performed.Results Compared with group S,the concentrations of Scr and plasma CD62P,renal Paller score and apoptosis rate of renal cells were significantly increased in Sep and T groups(P＜0.05).Compared with group Sep,the concentra-tions of Scr and plasma CD62P,renal Paller score and apoptosis rate of renal cells were significantly de-creased in group T(P＜0.05).Conclusion The mechanism by which ticagrelor reduces acute kidney injury is related to decreasing plasma CD62P concentrations and inhibiting cell apoptosis in septic rats.