Preparation of monoclonal antibodies against pneumococcal polysaccharide and hepatitis B virus sur-face protein / 中华微生物学和免疫学杂志

ABSTRACT

Objective To prepare monoclonal antibodies against pneumonia serotype 33F polysac-charides (Pn33Fps) and hepatitis B virus ( HBV) surface proteins ( HBs) by using the conjugate of Pn33Fps and HBs as antigen. Methods The conjugate of Pn33Fps and HBs was used as antigen to immu-nize mice with different immune doses, different immune procedures and different immune sites. Mouse spleen cells with higher antibody level after immunization were isolated and fused with SP2/0 myeloma cells. The hybridoma cells were screened specifically with Pn33Fps or HBs to prepare corresponding monoclonal antibodies. Results Serum antibodies against Pn33Fps and HBs were induced by immunizing mice with the conjugate. Monoclonal cell lines capable of continuously expressing antibodies against Pn33Fps or HBs were obtained. It has been proved that the recovery rates of samples of Pn33Fps and HBs prepared in three bat-ches tested with ascites monoclonal antibodies prepared by these two monoclonal cell lines were between 95％ and 105％. Conclusions Monoclonal antibodies against Pn33Fps and HBs could be prepared simul-taneously by immunizing mice with the conjugate of Pn33Fps and HBs and used for the quantitative detection of Pn33Fps and HBs.