Simultaneous determination of multiple constituents of QingJinHuaTan Decoction by ultra high performance liquid chromatography / 药学学报
Acta Pharmaceutica Sinica
; (12): 1872-1876, 2020.
Article
em Zh
| WPRIM
| ID: wpr-825158
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WPRO
ABSTRACT
A UHPLC method for the simultaneous determination of multiple constituents in QingJinHuaTan Decoction was established. The separation was performed on a Waters cortecs T3 column (150 mm×2.1 mm, 1.6 μm); the mobile phase was acetonitrile-water (containing 0.04% phosphoric acid) with gradient elution at a flow rate of 0.30 mL·min-1, the column temperature at 25 ℃ and the wavelengths at 238 nm and 280 nm. The results showed that all peaks were well separated and all components had a good linear relationship in the investigative range, (r > 0.999). The repeatability and stability were good and the recovery was between 92.5%-104.7%. The method is simple, accurate and reliable and provides a basis for quality control of QingJinHuaTan Decoction and for further development of methods for its standardization.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2020
Tipo de documento:
Article