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Study on the Improvement Effects of Sijunzi Decoction on Chronic Kidney Disease-protein Energy Wasting Model Mice and Its Mecranism / 中国药房
China Pharmacy ; (12): 2358-2364, 2020.
Artigo em Chinês | WPRIM | ID: wpr-825891
ABSTRACT
OBJECTIVE:To study the improvement effects of Sijunzi decoction on skeletal muscle atrophy in chronic kidney disease-protein energy wasting (CKD-PEW)model mice ,and to explore its potential mechanism . METHODS :A total of 80 mice were randomly divided into sham operation group (n=10)and modeling group (n=70). CKD-PEW model was established by removing 5/6 kidneys and giving a low-protein diet (4% casein)for mice in modeling group. Totally 50 modeled mice were randomly divided into model group ,Sijunzi decoction low-dose ,medium-dose and high-dose groups [ 2.34,4.68,9.36 g/(kg·d), by crude drug] ,Compound α-ketoacid tablets group [positive control ,1 g/(kg·d)],with 10 mice in each group. Administration groups were given relevant medicine intragastrically ;sham operation group and model group were given constant volume of normal saline intragastrically ,once a day ,for consecutive 14 d. After last medication ,body weight of mice and wet mass of left tibialis anterior muscle (TA)were weighed ;TA cross-sectional area was determined ;protein synthesis and decomposition metabolism ability of TA were detected ;mRNA expressions of Bcl-2,Bax and Caspase-3 in TA were detected by Real-time PCRprotein expressions of muscular dystrophin Fbox- 1 (Atrogin-1),myofloin-1 (MuRF-1),Rho-related protein kinase 1 (ROCK1), phosphorylated PTEN (p-PTEN),phosphatidylinositol-3-kinase(PI3K)and phosphorylated Akt (p-Akt)in TA were detected by Western blotting. RESULTS :Compared with the sham operation group ,the body weight ,TA wet weight ,protein synthesis metabolism ability of TA as well as protein expressions of PI 3K and p-Akt were decreased significantly in model group (P<0.05); the cross-sectional area of TA decreased significantly (P<0.05);protein decomposition metabolism ability of TA ,Bax/Bcl-2 ratio, Caspase-3 mRNA expression ,protein expressions of Atrogin- 1,MuRF-1,ROCK1 and p-PTEN were increased significantly (P< 0.05). Compared with model group ,above indexes of mice were all improved significantly in Sijunzi decoction medium-dose , high-dose groups and Compound α-ketoacid tablets group (P<0.05). CONCLUSIONS :Sijunzi decoction can increase the body weight of CKD-PEW model mice and alleviate the skeletal atrophy ;the mechanism may be related to regulating ROCK 1/PTEN/Akt signaling pathway activity ,inhibiting the expression of Atrogin- 1 and MuRF- 1.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2020 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2020 Tipo de documento: Artigo