Effects of Cyclic Nucleotide-Gated Channels in Vestibular Nuclear Neurons / 전남의대학술지
Chonnam Medical Journal
;
: 155-159, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-82693
ABSTRACT
This study was designed to investigate the effects an 8-Br-cGMP on the neuronal activity of rat vestibular nuclear cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated vestibular nuclear cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium currents were recorded by standard patch-clamp techniques under current and voltage-clamp modes. Twelve vestibular nuclear cells revealed excitatory responses to 1-5 microM of 8-Br-cGMP, and 3 neurons did not respond to 8-Br-cGMP. Whole potassium currents of vestibular nuclear cells were decreased by 8-Br-cGMP (n=12). After calcium-dependent potassium currents were blocked by tetraethylammonium, the potassium currents were not decreased by 8-Br-cGMP. These experimental results suggest that 8-Br-cGMP changes the neuronal activity of vestibular nuclear cells by blocking the calcium-dependent potassium currents that underlie the afterhyperpolarization.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Potássio
/
Potenciais de Ação
/
Pronase
/
Termolisina
/
Ratos Sprague-Dawley
/
Técnicas de Patch-Clamp
/
Tetraetilamônio
/
Éter
/
Anestesia
/
Neurônios
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Chonnam Medical Journal
Ano de publicação:
2011
Tipo de documento:
Artigo
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