AFF4 regulates osteogenic differentiation of human dental follicle cells / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 20-20, 2020.
Artigo
em Inglês
| WPRIM
| ID: wpr-828958
ABSTRACT
As a member of the AFF (AF4/FMR2) family, AFF4 is a transcription elongation factor that is a component of the super elongation complex. AFF4 serves as a scaffolding protein that connects transcription factors and promotes gene transcription through elongation and chromatin remodelling. Here, we investigated the effect of AFF4 on human dental follicle cells (DFCs) in osteogenic differentiation. In this study, we found that small interfering RNA-mediated depletion of AFF4 resulted in decreased alkaline phosphatase (ALP) activity and impaired mineralization. In addition, the expression of osteogenic-related genes (DLX5, SP7, RUNX2 and BGLAP) was significantly downregulated. In contrast, lentivirus-mediated overexpression of AFF4 significantly enhanced the osteogenic potential of human DFCs. Mechanistically, we found that both the mRNA and protein levels of ALKBH1, a critical regulator of epigenetics, changed in accordance with AFF4 expression levels. Overexpression of ALKBH1 in AFF4-depleted DFCs partially rescued the impairment of osteogenic differentiation. Our data indicated that AFF4 promoted the osteogenic differentiation of DFCs by upregulating the transcription of ALKBH1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Proteínas Repressoras
/
Fatores de Transcrição
/
Biomarcadores
/
Diferenciação Celular
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
Fatores de Elongação da Transcrição
/
Saco Dentário
/
Genética
Limite:
Humanos
Idioma:
Inglês
Revista:
International Journal of Oral Science
Ano de publicação:
2020
Tipo de documento:
Artigo
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