Differential gene expression of transglutaminase 2 in term pregnancy with and without labor / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 510-517, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-83069
ABSTRACT
OBJECTIVE:
Our purpose was to investigate transglutaminase 2 (TGM2) mRNA and protein expressions in term placentas and fetal membranes delivered with labor compared to no labor.METHODS:
Samples were obtained from five cases delivered with labor and five cases delivered without labor after 38 weeks of gestation. Each sample was collected from amnion, chorion, central and peripheral portion of the basal plate of placenta. Real time polymerase chain reaction (RT-PCR) was done to analyze mRNA expression of TGM2. Western blot was done and TGM2 protein level was detected. Mann-Whitney U test was used for statistic analysis.RESULTS:
In labor group, TGM2 mRNA expressions were decreased compared to no labor group in 3 sampling sites except chorion (0.66+/-0.10 vs 1.29+/-0.12 in peripheral placenta, 0.67+/-0.23 vs 1.02+/-0.02 in central placenta, 0.70+/-0.16 vs 1.04+/-0.05 in amnion in contrast with 1.62+/-0.64 vs 1.56+/-0.21 in chorion). TGM2 protein expressions of four differential portions were decreased in all labor groups (1.05+/-0.35 vs 1.27+/-0.19 in peripheral placenta, 0.69+/-0.84 vs 0.84+/-0.31 in central placenta, 0.33+/-0.15 vs 0.39+/-0.33 in amnion, 0.96+/-0.18 vs 1.77+/-0.61 in chorion).CONCLUSIONS:
This result suggests that TGM2 might involve in labor progress of term pregnancy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
RNA Mensageiro
/
Expressão Gênica
/
Transglutaminases
/
Western Blotting
/
Córion
/
Proteínas de Ligação ao GTP
/
Membranas Extraembrionárias
/
Reação em Cadeia da Polimerase em Tempo Real
/
Âmnio
Limite:
Gravidez
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2008
Tipo de documento:
Artigo
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