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Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1beta in RAW 264.7 cells / 대한마취과학회지
Korean Journal of Anesthesiology ; : 161-165, 2012.
Artigo em Inglês | WPRIM | ID: wpr-83303
ABSTRACT

BACKGROUND:

Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages.

METHODS:

RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 microM) manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1beta were determined 30 min and 24 h after the addition of LPS respectively.

RESULTS:

Manassantin B inhibited the production of IL-1beta and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS.

CONCLUSIONS:

Manassantin B reduces LPS-induced IL-1beta expression through effects on ERK1/2- and p38 MAPK-mediated pathways. Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Processos Patológicos / Fosforilação / Fosfotransferases / Proteínas Quinases / Choque Séptico / Capilares / Lipopolissacarídeos / Citocinas / Interleucinas / Sepse Idioma: Inglês Revista: Korean Journal of Anesthesiology Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Processos Patológicos / Fosforilação / Fosfotransferases / Proteínas Quinases / Choque Séptico / Capilares / Lipopolissacarídeos / Citocinas / Interleucinas / Sepse Idioma: Inglês Revista: Korean Journal of Anesthesiology Ano de publicação: 2012 Tipo de documento: Artigo