Effects of magnesium sulfate on VEGF and Caspase-3 of HUVECs (Human umbilical vein endothelial cells) / 대한주산의학회잡지
Korean Journal of Perinatology
; : 262-267, 2004.
Article
em Ko
| WPRIM
| ID: wpr-83414
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVES: To determine the effects of magnesium sulfate on Caspase-3 and Vascular endothelial growth factor (VEGF) of human umbilical vein endothelial cells (HUVECs) under presence or absence of Tumor necrosis factor (TNF-alpha). METHODS: HUVECs were isolated from normal term umbilical cords and cultured in several physiolo gically relevant concentrations of magnesium sulfate with or without exposure of TNF-alpha. The concentrations of VEGF and caspase-3 were estimated by colorimetric assay and ELISA assay, respectively. RESULTS: The concentration of VEGF in HUVECs significantly increased in the presence of TNF-alpha compared with in the absence of TNF-alpha. However, the concentration of VEGF did not show significant difference in several concentrations of magnesium sulfate concentrations with addition of TNF-alpha and it showed the lowest concentration under 4 mM and 8 mM of magnesium sulfate concentration without addition of TNF-alpha. The concentration of caspase-3 in HUVECs did not show statistically significant difference with the addition of TNF-alpha and magnesium sulfate. CONCLUSION: TNF-alpha induce HUVECs to stimulate the VEGF, and magnesium sulfate might not have the effects on the expression of VEGF with addition of TNF-alpha However, the concentration for treatment of magnesium sulfate inhibits the expression of VEGF without addition of TNF-alpha. Magnesium sulfate might not have an effect of the expression of caspase-3 with or without addition of TNF-alpha.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Cordão Umbilical
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Veias Umbilicais
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Ensaio de Imunoadsorção Enzimática
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Fator de Necrose Tumoral alfa
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Fator A de Crescimento do Endotélio Vascular
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Caspase 3
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Células Endoteliais da Veia Umbilical Humana
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Magnésio
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Sulfato de Magnésio
Idioma:
Ko
Revista:
Korean Journal of Perinatology
Ano de publicação:
2004
Tipo de documento:
Article