Modified Dextrose Starch Agar for the Preservation of Neisseria gonorrhoeae
Yonsei Medical Journal
;
: 70-74, 1978.
Artigo
em Inglês
| WPRIM
| ID: wpr-8344
ABSTRACT
To seek a practical and inexpensive method to preserve gonococcal cultures, a few methods were compared. The following methods kept the cultures alive for only a short period of time skim milk at -20 degrees C; tryptic soy broth with 15% glycerol at -20 degrees C; cystine tryptic agar at 35 degrees C. Most of the test cultures survived for more than 4 weeks in the following media one half strength dextrose starch agar; one half strength dextrose starch agar with ferric nitrate; one half strength dextrose starch agar with antimicrobic CNV. Dextrose starch agar could be substituted by GC medium base with a slight modification. It is concluded that preservation of gonococci in one half strength dextrose starch agar with CNV, which produces less frequent contamination, is a practical method to maintain cultures for teaching and quality control.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Preservação Biológica
/
Amido
/
Técnicas Bacteriológicas
/
Meios de Cultura
/
Ágar
/
Glucose
/
Neisseria gonorrhoeae
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
1978
Tipo de documento:
Artigo
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