Population dynamics of the mood-sucking insects and detection of pathogens in the rodents from xisha islands of china / 第二军医大学学报

ABSTRACT

Objective To investigate the popukton dynamics of blood-sucking insects and detect the pathogen of rodents in Xisha Islands of China, so as to provide information for prevention and control of infectious diseases in the area. Methods From January to July in 2014. we collected blood-sucking msccis in Yongxing and Shi Islands twice a month using light traps. The specimens were identified by morphologic characters and molecular markers. Meanwhile, the population size was calculated. The pathogenic infections of rodints were detected by immuno-collcadal gold chromatographic test stnps and PCR assay. And the ectoparasates in the rodents were initially identified by morphologic characters. Krsilts The dominant species of blood-sucking insects in Xisha Islands were species of Genus Cutex, Armine res and Cuticoides, and in Apnl the population density of biting midge was the highest (55. 55%. 6 984/12 573). Phlebotomine sandfly specimen was also collected and identified as Sergentomyia baily (n= 11) by mtDNA-CO I sequences. The positive rates of Staphylococcus aureus aiterotoxin type A and Botulinum toxin type A were 3. 45% (1/29) and 14.00% (7/50) in the rodent serum samples, while all the samples were negative for Tsutsugamushi disease and Plague antibodies. Srta58 gene of Rickettsia tsutsugamushi was amplified by nested FCR m 70 rodent’s spkren tissues, with the positive rate bang 11. 43% (8/70). A total of 248 ectoparasites were collected, and 93.55% (232/248) of them was gamasid mites. Conclusion The population dynamics of blood-sucking insects has no obvious change. Phtebotamme sandflies has been first recorded in Xisha Islands. The infection rates by Rickettsia tsutsugaimslx i and two bacterial toxins are high.