Your browser doesn't support javascript.
loading
Correlation between ubiquitin specific peptidase 7 expression and male infertility / 第二军医大学学报
Academic Journal of Second Military Medical University ; (12): 986-991, 2016.
Artigo em Chinês | WPRIM | ID: wpr-838720
ABSTRACT
Objective To investigate the expression of ubiquitin specific peptidase 7 (USP7) in the sperm of infertile man suffering asthenozoospermia or oligoasthenozoospermia, and to explore its role in the pathogenesis of male infertility. Methods The semen samples were obtained from 120 donors attending the Fertility Clinic, the Centre of Reproductive Medicine, the Second Affiliated Hospital, Chongqing Medical University. The sperm specimens were divided into normozoospermia group (n=57), asthenozoospermia group (n =37) or oligoasthenozoospermia group (n =26) after semen analysis according to 2010 WHO standards. The localization and expression of USP7 were examined using immunofluorescence and Western blotting analysis in the three groups. Then the correlation between USP7 expression and sperm parameterswas analyzed. Results USP7 mainly localized in the middle part and main part of thesperm tail; the USP expression was significantly lower in asthenozoospermia group(0. 86 ± 0. 53) and oligoasthenozoospermia group (0. 62 ± 0. 43) compared with normozoospermia group (1. 63 ± 0. 76, P<0. 01). And USP7 expression was found positively correlated with the ratio of sperm moving forward(r = 0. 431 2, P =0. 008 7), total motility rate(r=0. 443 8, P =0. 006 7), and sperm concentration(r = 0. 455 0, P =0. 005 3). Conclusion The lowered expression of USP7 is related to the reduction of human sperm motility and concentration, indicating that USP7 expressionmay serve as an indicator for sperm quality evaluation in infertile men.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Guia de Prática Clínica Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 2016 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Guia de Prática Clínica Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 2016 Tipo de documento: Artigo