An animal model of open craniocerebral injury with seawater immersion / 第二军医大学学报

ABSTRACT

Objective To establish an animal model of open craniocerebral injury with seawater immersion and to study the characteristics of the injury. Methods A total of 100 healthy ma! SD rats were divided into 5 groups control group, mild injury + seawater immersion group, moderate injury + seawater immersion group, severe injury + seawater immersion group and severe injury + normal saline immersion group. After SD rats had received a controlled cortical impact (CCI) injury, the injured brain tissue was soaked in seawater or normal saline to construct an animal model of open craniocerebral injury. We then observed the characteristics of the model, including the animal behavior, pathological changes, brain water content and the levll of blood brain barrier (BBB) opening. Results The animal model could well simulate the characteristics of the open craniocerebral injury with seawater immersion. With the increase of brain injury severity, the cerebral dysfunction, cerebral pathology, cerebral water content, and the damage of BBB became more serious. The pathological changes of brain tissue necrosis and bleeding in severe injury + seawater immersion group were more nota Me than those in the severe injury + normal saline immersion group. Compared with the severe injury + saline immersion group, the water content of brain tissue and the damage of BBB were significantly increased in the severe injury+ seawater immersion group after immersion for 24 h. Conclusion The rat model we established in this study can simulate the open craniocerebral injury with seawater immersion.