HPLC-DAD for simultaneous determination of 20 colorants in hair dyes / 第二军医大学学报

ABSTRACT

Objective: To develop a high-performance liquid chromatography (HPLC)-diode array detector (DAD) method for determination of 20 colorants in hair dyes, so as to improve the method for determining oxidative hair dyes in Hygienic Standard for Cosmetics. Methods: After ultrasonic extraction with ethanol-water, samples were analyzed by HPLC. The separation was achieved on an Agilent Zorbax Bonus-RP column (4.6mm × 250mm, 5μm) using a gradient mobile phase system of A acetonitrile and B 4mmol/L PBS (pH = 6.0) containing 0.1 % 1-octanesulfonic acid sodium salt solution at a flow rate of 1 mL/min. The gradient elution program was as follows 0-24 min, 10% A; 24-26 min, 10%-40% A; 26-45 min, 40% A; 45-47 min, 40%-80% A; 47-52 min, keep 80% A unchanged. The detection wavelength was set at 280 nm, the temperature of column was 15°C, and the injection volume was 5 μL. Results: All the components were separated at baseline within 50 min. Within the concentration range of 10.0-500.0 mg/L, the peak areas and concentrations of colorants had good linear relationship, with the linear correlation coefficients being higher than 0.999. The intra-day and inter-day precisions were within 4% and the average recoveries ranged from 81.90% to 119.88%. Conclusion: The present method is simple, rapid and accurate and is suitable for determination of colorants in oxidative hair dyes.