Transplantation of autologous angiogenin-overexpressing marrow stromal cells in treatment of a porcine chronic ischemia model / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 586-591, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-839151
ABSTRACT
Objective:
To investigate the effect of autologous angiogenin(ANG)-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia.Methods:
Porcine models of chronic ischemia were established and were randomized into the following 3 groups autologous marrow stromal cells transfected with Ad-ANG (Group I, n=11), autologous marrow stromal cells without transfection (Group II, n=10), and controls injected with serum-free DMEM medium (Group III, n=10). The autologous marrow stromal cells were cultured and were transfected with Ad-ANG, and the cells were labeled by CM-Dil. The cels were transplanted to the ischemia site of porcine heart. The parameters we observed included Rentrop score, ejection fraction, percentage of infracted area, CM-Dil labeled cells under fluorescence microscope, number of blood vessels and expression of ANG protein.Results:
Compared with group H and IE, Group I had significantly increased Rentrop score, ejection fraction, numbers of blood vessels in the infarcted areas, CM-DiI labeled cells, and ANG protein expression, but significantly decreased infarcted area (P < 0. 05 or P < 0. 01).Conclusion:
Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells, greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo prognóstico
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo
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