LC-MS/MS in determination of Bletilla striata glucomannan in Blettila striata hemostatic sponge / 第二军医大学学报

ABSTRACT

Objective To establish a LC-MSIMS system for the determination of Bletilla striata glucomannan in Bletilla striata hemostatic sponge. Methods The Waters X Bridge™ Amide(3. 5 [J-ill, 2. 1 mm X 100 mm) column was used at 35 'C; acetonitrile and 5 mmol/L ammonium acetate (containing 0.1% formic acid) (60 40, VIV) were used as the mobile phase at a flow rate of 0. 3 mLimin, with the injection volume being 10 [J-L. Results The linear range for D-mannose was 50. 0-5 000. 0 nglmL (r=O. 999 5). The results of intra-day and inter-day precisions, limit of detection and limit of quantitation were all within the normal ranges. The recovery rate (n=5) was 98. 4%, RSD= 1. 03%. Conclusion The method in this study is simple, selective and sensitive; it can be used for the quantitative determination and quality control of Bletilla striata glucomannan.