Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1151-1154, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-839492
ABSTRACT
Objective:
our study was to investigate the effect of hepaCAM gene on cell cycle of bladder cancer cell line T24 and BIU-87.Method:
Adenovirus vector with hepaCAM was infected into T24 and BIU-87 cells. The expression of hepaCAM mRNA and protein were measured byRT-QPCR and Western-blot. Immunofluorescence detected the cellular localization of hepaCAM. The distribution of cell cycle was analyzed by FCM. The protein expression of cyclinD1 was measured by Westein-blot.Result:
The expression of hepaCAM mRNA and protein was increased in two cell lines infected with pAdH5-hepaCAM. The G0/G1 cycle was increased in infected cells, and the protein expression of cyclinD1 was decreased in same cells. Immunofluorescence revealed that hepaCAM protein localized on cytoplasm in well-spread cells, otherwise it localized on the junction of each cell.Conclusion:
T24 and BIU-87 cells that infected pAdH5- hepaCAM was blocked in G0/G1 cycle, and it can decreased the protein expression of cyclinD1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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