Isosorbide mononitrate upregulates platelet-derived growth factor receptor β and promotes angiogenesis in zebrafish / 第二军医大学学报

ABSTRACT

Objective To investigate the pro-angiogenesis mechanism of isosorbide mononitrate by observeing its effect on the angiogenesis in Tg(flk1EGFP) transgenic zebrafish. Methods The embryos incubated with isosorbide mononitrate (100, 300, 500, and 1 000 μg/ml) were taken as the experiment groups, and the embryos exposed to 0. 5% DMSO were identified as the controls. The embryos were treated one hour before the postfertilization stage, and the status and length of the newly- formed blood vesseis were observed under fluorescence microscope 24-26 h after treatments. The total RNAs were extracted from zebrafish embryos at 0, 6, 12, and 24 h after treatment; real-time quantitative RT-PCR was performed to examine the ievel of Platelet-derived growth factor receptor β(PDGFRβ). Resutts Isosorbide mononitrate greatly promoted the growth of the intersegmental vessels in zebrafish (P<0. 01). It also dose-dependently promoted the growth of vascular at concentrations 100, 300, and 500 μg/ml. The expression levels of PDGFRβ gene were increased in the experiment groups compared with DMSO group, with significant difference found at 12 h and 24 h after treatment (P<0. 05, P<0. 01). Conclusion Our data suggest that isosorbide mononitrate can up-regulate PDGFRf expression and promote angiogenesis in zebrafish.