Inhibitory effect of recombinant adenovirus expressing TEM8 gene against angiogenesis in mouse model of glioma / 第二军医大学学报

ABSTRACT

Objective: To evaluate the inhibitory effects of recombinant adenovirus carrying TEM8 (Ad-TEM8) against angiogenesis and growth of mouse glioma G422 cells in vitro and in vivo. Methods: The Ad-TEM8 was constructed and subcutaneously injected to immunize Kunming mice, and then the splenic lymphocytes were collected for co-culture with G422 cells in vitro. The growth inhibition of G422 cells was observed and IFN-γ secretion by the activated lymphocytes were examined by ELISOT method. In addition, the anti-tumor and anti-angiogenesis effects ofAd-TEM8 were observed in Kunming mice carrying G422 xeno grafts. Results: Ad-TEM8 effectively stimulated the generation of cytotoxic Tlymphocytes (CTLs)in Kunming mice and the secretion of IFN-γ. Subcutaneous injection of Ad-TEM8-induced cytotoxicity effectively inhibited in vivo tumor growth and angiogenesis. Conclusion: Ad-TEM8 can induce CTLs in mice, inhibit tumor growth in vitro and in vivo, and disrupt tumor vasculature. The anti-angiogenesis effect might be a mechanism for its in vivo anti-tumor effect; and TEM8 may serve as a novel target for anti-angiogenesis therapy of glioma.